Page last modified May 22, 2005
Last minor edit May 23, 2007
This egg of the Icaricia lupini butterfly ("Lupine
Blue"), was processed by Helicon
Focus 3.10 from two extended-depth-of-field stacks. The egg
diameter is approximately 0.5 mm. The views shown here are what
you would see through a 100X microscope, except that with a 100X
microscope you wouldn't get stereo and you can't see the whole egg in
focus at once.
First, for those who can't see stereo, here is a "rocking" version.
OK, here's the stereo version. Viewing format is crossed-eye: left-eye image on the right, right-eye image on the left.
The two image stacks were shot by moving the camera, not the subject. It is important that the subject be lit identically in both views, and I find that it's easier to move the camera than to move the subject and lighting in tandem. To determine the separation, I simply positioned my personal head so that my right eye was looking along the optical axis, then while holding my head in place, moved the camera so that my left eye was looking along the optical axis. I don't know the exact separation; probably around 6 degrees.
Original stacks shot with a 10X, 0.25 N.A. microscope objective on 240 mm tube, advancing focus by 1/3000 inch per step. Further information about the photographic setup can be found at http://www.janrik.net/insects/ExtendedDOF/index.html
The above stereo pair can be viewed in high resolution using StereoPhoto Maker (SPM), via right-click and 'Open with SPM'. (Be sure to use Internet Explorer, and setup SPM using 'Edit/Preferences/IE Context Menu', check 'Enable IE Context Menu'.)
In case you're wondering, here is the extended depth of field image paired with one of the source images (which is what you'd see through the microscope).
Claims and Disclaims
The work presented here is a personal publication of capabilities and
techniques developed by the author. You may link to this page,
but please do not reference in archival publications; contact the
author instead. All images and text
are copyright Rik Littlefield, 2005.
First, for those who can't see stereo, here is a "rocking" version.
OK, here's the stereo version. Viewing format is crossed-eye: left-eye image on the right, right-eye image on the left.
The two image stacks were shot by moving the camera, not the subject. It is important that the subject be lit identically in both views, and I find that it's easier to move the camera than to move the subject and lighting in tandem. To determine the separation, I simply positioned my personal head so that my right eye was looking along the optical axis, then while holding my head in place, moved the camera so that my left eye was looking along the optical axis. I don't know the exact separation; probably around 6 degrees.
Original stacks shot with a 10X, 0.25 N.A. microscope objective on 240 mm tube, advancing focus by 1/3000 inch per step. Further information about the photographic setup can be found at http://www.janrik.net/insects/ExtendedDOF/index.html
The above stereo pair can be viewed in high resolution using StereoPhoto Maker (SPM), via right-click and 'Open with SPM'. (Be sure to use Internet Explorer, and setup SPM using 'Edit/Preferences/IE Context Menu', check 'Enable IE Context Menu'.)
In case you're wondering, here is the extended depth of field image paired with one of the source images (which is what you'd see through the microscope).